Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Review (1) Publications (86) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at 1:18000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at 1:18000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Immunofluorescence analysis of paraffin-embedded mouse brain using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-p38 MAPK-T180/Y182 Rabbit pAb(AP0526)

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Overview

Product name Phospho-p38 MAPK-T180/Y182 Rabbit pAb
Catalog No. AP0526
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T180 & Y182 of human p38 MAPK (NP_620581.1).
Sequence MTGYV
Gene ID 5600630056031432
Swiss prot Q15759P53778O15264Q16539
Synonyms MAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK; Phospho-p38 MAPK-T180/Y182
Calculated MW 29kDa/34kDa/35kDa/41kDa
Observed MW 43kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:20000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T, NIH/3T3
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Mouse embryonal fibroblast cellHomo sapiens, Mus musculus, Rattus norvegicus, Homo sapiens, Danio rerio, Sus scrofa, Melodinus cochinchinensis, floral, Gallus gallus, Bos taurus, Crayfish, Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Phospho-p38 MAPK-T180/Y182 Rabbit pAb images

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)}

Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at 1:18000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)}

Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at 1:18000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunofluorescence - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)}

Immunofluorescence - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Immunofluorescence analysis of paraffin-embedded mouse brain using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPK14. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK14. (Distance between topics and target gene indicate popularity.) MAPK14

* Data provided by citexs.com, for reference only.

Publishing research using AP0526? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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