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Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Reviews (2)Publications (28) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 antibody (AP0526) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

Immunofluorescence analysis of mouse brain using Phospho-p38 MAPK-T180/Y182 antibody (AP0526) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-p38 MAPK-T180/Y182 Rabbit pAb(AP0526)
Review
ABclonal: review for Phospho-p38 MAPK-T180/Y182 Rabbit pAb(AP0526)

Overview

Product namePhospho-p38 MAPK-T180/Y182 Rabbit pAb
Catalog No.AP0526
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
ImmunogenA synthetic phosphorylated peptide around T180 & Y182 of human p38 MAPK (NP_620581.1).
SequenceMTGYV
Gene ID5600630056031432
Swiss protQ15759P53778O15264Q16539
SynonymsMAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK
Calculated MW29kDa/34kDa/35kDa/41kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesNIH/3T3
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse embryonal fibroblast cell, Cortical neurons)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Danio rerio, Sus scrofa, Melodinus cochinchinensis, floral)

    ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) }

    Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

    Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 antibody (AP0526) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) }

    Immunofluorescence - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526)

    Immunofluorescence analysis of mouse brain using Phospho-p38 MAPK-T180/Y182 antibody (AP0526) at dilution of 1:100. Blue: DAPI for nuclear staining.

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