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Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Reviews (4)Publications (7) Datasheet

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Western blot analysis of extracts of HeLa cells, using Phospho-MAPK14-T180/Y182 pAb (AP0297) at 1:2000 dilution or MAPK14 antibody (A14401).HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for Phospho-p38 MAPK-T180/Y182 Rabbit pAb(AP0297)
Review
ABclonal: review for Phospho-p38 MAPK-T180/Y182 Rabbit pAb(AP0297)
Review
ABclonal: review for Phospho-p38 MAPK-T180/Y182 Rabbit pAb(AP0297)
Review
ABclonal: review for Phospho-p38 MAPK-T180/Y182 Rabbit pAb(AP0297)

Overview

Product namePhospho-p38 MAPK-T180/Y182 Rabbit pAb
Catalog No.AP0297
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
ImmunogenA synthetic phosphorylated peptide around T180 & Y182 of human p38 MAPK (NP_620581.1).
SequenceMTGYV
Gene ID1432
Swiss protQ16539
SynonymsMAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK
Calculated MW29kDa/34kDa/35kDa/41kDa
Observed MW41kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
Customer validation

(mouse cells, HepG2)

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297) }

Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Western blot analysis of extracts of HeLa cells, using Phospho-MAPK14-T180/Y182 pAb (AP0297) at 1:2000 dilution or MAPK14 antibody (A14401).HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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