Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-mTOR-S2448 Rabbit pAb (AP0094)

Review (1)Publications (23) Datasheet

ABclonal:Western blot - Phospho-mTOR-S2448 Rabbit pAb (AP0094)

Western blot analysis of extracts of 293T cells, using Phospho-mTOR-S2448 antibody (AP0094) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Phospho-mTOR-S2448 Rabbit pAb (AP0094)

Immunofluorescence analysis of HeLa cells using Phospho-MTOR-S2448 antibody (AP0094) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-mTOR-S2448 Rabbit pAb(AP0094)

Overview

Product namePhospho-mTOR-S2448 Rabbit pAb
Catalog No.AP0094
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
ImmunogenA synthetic phosphorylated peptide around S2448 of human mTOR (NP_004949.1).
SequenceTDSYS
Gene ID2475
Swiss protP42345
SynonymsFRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mTOR; MTOR
Calculated MW288kDa
Observed MW289KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples293T
Cellular locationCytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein
Customer validation

(H460)

WB(Macaca mulatta, Homo sapiens, Sus scrofa, Rattus norvegicus, Mus musculus, Capra hircus)

ABclonal:Western blot - Phospho-mTOR-S2448 Rabbit pAb (AP0094) }

Western blot - Phospho-mTOR-S2448 Rabbit pAb (AP0094)

Western blot analysis of extracts of 293T cells, using Phospho-mTOR-S2448 antibody (AP0094) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - Phospho-mTOR-S2448 Rabbit pAb (AP0094) }

Immunofluorescence - Phospho-mTOR-S2448 Rabbit pAb (AP0094)

Immunofluorescence analysis of HeLa cells using Phospho-MTOR-S2448 antibody (AP0094) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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