Western blot analysis of extracts of 293T cells, using Phospho-mTOR-S2448 antibody (AP0094) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunofluorescence analysis of HeLa cells using Phospho-MTOR-S2448 antibody (AP0094) at dilution of 1:100. Blue: DAPI for nuclear staining.
|Product name||Phospho-mTOR-S2448 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
IF 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunofluorescence|
|Cellular location||Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein|
WB(Macaca mulatta, Homo sapiens, Sus scrofa, Rattus norvegicus, Mus musculus, Capra hircus)
* For research use only. Not for therapeutic or diagnostic purposes.
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