Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-eNOS-S1177 Rabbit pAb (AP0421)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-eNOS-S1177 Rabbit pAb (AP0421)

Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit pAb (AP0421).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Western blot - Phospho-eNOS-S1177 Rabbit pAb (AP0421)

Western blot analysis of lysates from HeLa cells, using Phospho-eNOS-S1177 Rabbit pAb (A1548).HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

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Overview

Product name Phospho-eNOS-S1177 Rabbit pAb
Catalog No. AP0421
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).
Sequence TQSFS
Gene ID 4846
Swiss prot P29474
Synonyms eNOS; ECNOS; Phospho-eNOS-S1177
Calculated MW 133kDa
Observed MW 133kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat, HeLa
Cellular location Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-eNOS-S1177 Rabbit pAb images

ABclonal:Western blot - Phospho-eNOS-S1177 Rabbit pAb (AP0421)}

Western blot - Phospho-eNOS-S1177 Rabbit pAb (AP0421)

Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit pAb (AP0421).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
ABclonal:Western blot - Phospho-eNOS-S1177 Rabbit pAb (AP0421)}

Western blot - Phospho-eNOS-S1177 Rabbit pAb (AP0421)

Western blot analysis of lysates from HeLa cells, using Phospho-eNOS-S1177 Rabbit pAb (A1548).HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

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Related Targets

Based on existing publications, the following genes are closely related to research on NOS3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NOS3. (Distance between topics and target gene indicate popularity.) NOS3

* Data provided by citexs.com, for reference only.

Publishing research using AP0421? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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