Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-eIF4EBP1-S65 Rabbit pAb (AP0032)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-eIF4EBP1-S65 Rabbit pAb (AP0032)

Western blot analysis of lysates from 293T cells using Phospho-eIF4EBP1-S65 Rabbit pAb (AP0032) at 1:700 dilution. 293T cells were treated by λPP mixed solution (1ul) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name Phospho-eIF4EBP1-S65 Rabbit pAb
Catalog No. AP0032
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1).
Sequence RNSPV
Gene ID 1978
Swiss prot Q13541
Synonyms BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-S65
Calculated MW 13kDa
Observed MW 15-20kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T
Cellular location cytoplasm, cytosol
Customer validation

WB (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-eIF4EBP1-S65 Rabbit pAb images

ABclonal:Western blot - Phospho-eIF4EBP1-S65 Rabbit pAb (AP0032)}

Western blot - Phospho-eIF4EBP1-S65 Rabbit pAb (AP0032)

Western blot analysis of lysates from 293T cells using Phospho-eIF4EBP1-S65 Rabbit pAb (AP0032) at 1:700 dilution. 293T cells were treated by λPP mixed solution (1ul) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on EIF4EBP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EIF4EBP1. (Distance between topics and target gene indicate popularity.) EIF4EBP1

* Data provided by citexs.com, for reference only.

Publishing research using AP0032? Please let us know so that we can cite the reference in this datasheet.

Antibodies (15)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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