Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-eIF2α-S51 Rabbit pAb (AP0745)

Reviews (2)Publication (1) Datasheet

ABclonal:Western blot - Phospho-eIF2α-S51 Rabbit pAb (AP0745)
ABclonal:Immunoprecipitation - Phospho-eIF2α-S51 Rabbit pAb (AP0745)
Review
ABclonal: review for Phospho-eIF2α-S51 Rabbit pAb(AP0745)
Review
ABclonal: review for Phospho-eIF2α-S51 Rabbit pAb(AP0745)

Overview

Product namePhospho-eIF2α-S51 Rabbit pAb
Catalog No.AP0745
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic phosphorylated peptide around S51 of human eIF2α (NP_004085.1).
SequenceSELSR
Gene ID1965
Swiss protP05198
SynonymsEIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1
Calculated MW36kDa
Observed MW38kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa
Cellular locationCytoplasmic granule
Customer validation

(293T cells, MSC cells)

WB(Mus musculus)

ABclonal:Western blot - Phospho-eIF2α-S51 Rabbit pAb (AP0745) }

Western blot - Phospho-eIF2α-S51 Rabbit pAb (AP0745)

Western blot analysis of extracts of HeLa cells, using Phospho-eIF2α-S51 pAb (AP0745) at 1:2000 dilution or EIF2S1 antibody (A0764).HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - Phospho-eIF2α-S51 Rabbit pAb (AP0745) }

Immunoprecipitation - Phospho-eIF2α-S51 Rabbit pAb (AP0745)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb (AP0745). Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb (AP0745) at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.

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