Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Western blot analysis of extracts of 293T cells, using Phospho-c-Myc-T58 antibody (AP0080) at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Immunohistochemistry analysis of paraffin-embedded rat testis using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Immunohistochemistry analysis of paraffin-embedded mouse testis using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

You may also interested in:

Overview

Product name Phospho-c-Myc-T58 Rabbit pAb
Catalog No. AP0080
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T58 of mouse MYC (NP_001170823.1).
Sequence LPTPP
Gene ID 4609
Swiss prot P01106
Synonyms MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-T58
Calculated MW 51kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T
Cellular location Nucleus, nucleolus, nucleoplasm
Customer validation

WB (Homo sapiens)

IF (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-c-Myc-T58 Rabbit pAb images

ABclonal:Western blot - Phospho-c-Myc-T58 Rabbit pAb (AP0080)}

Western blot - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Western blot analysis of extracts of 293T cells, using Phospho-c-Myc-T58 antibody (AP0080) at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)}

Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Immunohistochemistry analysis of paraffin-embedded rat testis using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)}

Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)}

Immunohistochemistry - Phospho-c-Myc-T58 Rabbit pAb (AP0080)

Immunohistochemistry analysis of paraffin-embedded mouse testis using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Inquire About This Product

Submit your question about AP0080 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on MYC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MYC. (Distance between topics and target gene indicate popularity.) MYC

* Data provided by citexs.com, for reference only.

Publishing research using AP0080? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order