Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Western blot analysis of extracts of C6 cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Western blot analysis of extracts of HeLa cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name Phospho-Vimentin-S83 Rabbit pAb
Catalog No. AP1119
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S83 of human VIM (NP_003371.2).
Sequence QDSVD
Gene ID 7431
Swiss prot P08670
Synonyms VIM; CTRCT30; HEL113; vimentin; Phospho-Vimentin-S83
Calculated MW 54kDa
Observed MW 57kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, NIH/3T3, C6
Cellular location Cytoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-Vimentin-S83 Rabbit pAb images

ABclonal:Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)}

Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)}

Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Western blot analysis of extracts of C6 cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)}

Western blot - Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Western blot analysis of extracts of HeLa cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on VIM. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VIM. (Distance between topics and target gene indicate popularity.) VIM

* Data provided by citexs.com, for reference only.

Publishing research using AP1119? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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