Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-VAV1-Y174 Rabbit pAb (AP0180)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Phospho-VAV1-Y174 Rabbit pAb (AP0180)

Western blot analysis of extracts from Jurkat cells, using phospho-VAV1-Y174 antibody (AP0180).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-VAV1-Y174 Rabbit pAb
Catalog No. AP0180
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding Y174 of human VAV1
Sequence Email for sequence
Gene ID 7409
Swiss prot P15498
Synonyms VAV; Phospho-VAV1-Y174
Calculated MW 98kDa
Observed MW 98kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat
Cellular location cell-cell junction, cytoplasm, cytosol, plasma membrane

Research Area

Phospho-VAV1-Y174 Rabbit pAb images

ABclonal:Western blot - Phospho-VAV1-Y174 Rabbit pAb (AP0180)}

Western blot - Phospho-VAV1-Y174 Rabbit pAb (AP0180)

Western blot analysis of extracts from Jurkat cells, using phospho-VAV1-Y174 antibody (AP0180).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on VAV1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VAV1. (Distance between topics and target gene indicate popularity.) VAV1

* Data provided by citexs.com, for reference only.

Publishing research using AP0180? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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