Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-VASP-S239 Rabbit pAb (AP0187)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-VASP-S239 Rabbit pAb (AP0187)

Western blot analysis of lysates from C6 cells, using Phospho-VASP-S239 Rabbit pAb (AP0187) at 1:1000 dilution.C6 cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-VASP-S239 Rabbit pAb (AP0187)

Immunohistochemistry analysis of Phospho-VASP-S239 in paraffin-embedded rat spleen using Phospho-VASP-S239 Rabbit pAb (AP0187) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-VASP-S239 Rabbit pAb (AP0187)

Immunohistochemistry analysis of Phospho-VASP-S239 in paraffin-embedded human placenta using Phospho-VASP-S239 Rabbit pAb (AP0187) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-VASP-S239 Rabbit pAb
Catalog No. AP0187
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S239 of human VASP (NP_003361.1).
Sequence KVSKQ
Gene ID 7408
Swiss prot P50552
Synonyms VASP; Phospho-VASP-S239
Calculated MW 40kDa
Observed MW 50kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples C6
Cellular location Cell junction, Cell projection, Cytoplasm, cytoskeleton, filopodium membrane, focal adhesion, lamellipodium membrane, tight junction

Documents

Certificate of Compliance

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Research Area

Phospho-VASP-S239 Rabbit pAb images

ABclonal:Western blot - Phospho-VASP-S239 Rabbit pAb (AP0187)}

Western blot - Phospho-VASP-S239 Rabbit pAb (AP0187)

Western blot analysis of lysates from C6 cells, using Phospho-VASP-S239 Rabbit pAb (AP0187) at 1:1000 dilution.C6 cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-VASP-S239 Rabbit pAb (AP0187)}

Immunohistochemistry - Phospho-VASP-S239 Rabbit pAb (AP0187)

Immunohistochemistry analysis of Phospho-VASP-S239 in paraffin-embedded rat spleen using Phospho-VASP-S239 Rabbit pAb (AP0187) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-VASP-S239 Rabbit pAb (AP0187)}

Immunohistochemistry - Phospho-VASP-S239 Rabbit pAb (AP0187)

Immunohistochemistry analysis of Phospho-VASP-S239 in paraffin-embedded human placenta using Phospho-VASP-S239 Rabbit pAb (AP0187) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on VASP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VASP. (Distance between topics and target gene indicate popularity.) VASP

* Data provided by citexs.com, for reference only.

Publishing research using AP0187? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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