Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-ULK1-S757 Rabbit pAb (AP0736)

Publications (8) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-ULK1-S757 Rabbit pAb (AP0736)

Western blot analysis of lysates from A-431 cells, using Phospho-ULK1-S757 Rabbit pAb (AP0736) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name Phospho-ULK1-S757 Rabbit pAb
Catalog No. AP0736
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S757 of human Phospho-ULK1-S757 (NP_003556.1).
Sequence TVGSP
Gene ID 8408
Swiss prot O75385
Synonyms ATG1; ATG1A; UNC51; hATG1; Unc51.1; Phospho-ULK1-S757
Calculated MW 113kDa
Observed MW 145kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A-431
Cellular location Cytoplasm, Preautophagosomal structure, cytosol
Customer validation

WB (Spodoptera frugiperda, Homo sapiens, Rattus norvegicus, Fathead minnow)

IHC (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-ULK1-S757 Rabbit pAb images

ABclonal:Western blot - Phospho-ULK1-S757 Rabbit pAb (AP0736)}

Western blot - Phospho-ULK1-S757 Rabbit pAb (AP0736)

Western blot analysis of lysates from A-431 cells, using Phospho-ULK1-S757 Rabbit pAb (AP0736) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ULK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ULK1. (Distance between topics and target gene indicate popularity.) ULK1

* Data provided by citexs.com, for reference only.

Publishing research using AP0736? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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