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Phospho-ULK1-S757 Rabbit pAb (AP0736)

Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human

ABclonal:Western blot - Phospho-ULK1-S757 Rabbit pAb (AP0736)

Western blot analysis of extracts of A-431 cells, using Phospho-ULK1-S757 antibody (AP0736) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-ULK1-S757 Rabbit pAb
Catalog No.AP0736
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1 via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. Phosphorylates FLCN, promoting autophagy. Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation.
ImmunogenA synthetic phosphorylated peptide around S757 of human Phospho-ULK1-S757 (NP_003556.1).
SequenceTVGSP
Gene ID8408
Swiss protO75385
SynonymsULK1; ATG1; ATG1A; UNC51; Unc51.1; hATG1; ULk1
Calculated MW150kDa
Observed MW145KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesA-431
Cellular locationCytoplasm, Preautophagosomal structure, cytosol
Customer validation

WB(Spodoptera frugiperda, Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - Phospho-ULK1-S757 Rabbit pAb (AP0736) }

Western blot - Phospho-ULK1-S757 Rabbit pAb (AP0736)

Western blot analysis of extracts of A-431 cells, using Phospho-ULK1-S757 antibody (AP0736) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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