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Phospho-ULk1-S757 Rabbit pAb (AP0736)

Publication (1) Datasheet

ABclonal:Western blot - Phospho-ULk1-S757 Rabbit pAb (AP0736)

Overview

Product namePhospho-ULk1-S757 Rabbit pAb
Catalog No.AP0736
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
ImmunogenA synthetic phosphorylated peptide around S757 of human ULk1 (NP_003556.1).
SequenceTVGSP
Gene ID8408
Swiss protO75385
SynonymsULK1; ATG1; ATG1A; UNC51; Unc51.1; hATG1; ULk1
Calculated MW150kDa
Observed MW150kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesA-431
Cellular locationCytoplasm, Preautophagosomal structure, cytosol
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - Phospho-ULk1-S757 Rabbit pAb (AP0736) }

Western blot - Phospho-ULk1-S757 Rabbit pAb (AP0736)

Western blot analysis of extracts of A431 cells, using Phospho-ULk1-S757 pAb (AP0736) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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