Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Tau-T181 Rabbit pAb (AP0399)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Tau-T181 Rabbit pAb (AP0399)

Western blot analysis of extracts from mouse brain tissue using Phospho-Tau-T181 antibody (AP0399).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-Tau-T181 Rabbit pAb
Catalog No. AP0399
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T181 of human Tau
Sequence Email for sequence
Gene ID 4137
Swiss prot P10636
Synonyms TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6; Phospho-Tau-T181
Calculated MW 79kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse brain
Cellular location Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, axon, cytoskeleton, cytosol

Research Area

Phospho-Tau-T181 Rabbit pAb images

ABclonal:Western blot - Phospho-Tau-T181 Rabbit pAb (AP0399)}

Western blot - Phospho-Tau-T181 Rabbit pAb (AP0399)

Western blot analysis of extracts from mouse brain tissue using Phospho-Tau-T181 antibody (AP0399).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPT. (Distance between topics and target gene indicate popularity.) MAPT

* Data provided by citexs.com, for reference only.

Publishing research using AP0399? Please let us know so that we can cite the reference in this datasheet.

Antibodies (26)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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