Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Tau-S262 Rabbit pAb (AP0397)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Tau-S262 Rabbit pAb (AP0397)

Western blot analysis of lysates from Mouse brain, using Phospho-Tau-S262 Rabbit pAb (AP0397) at 1:500 dilution.Mouse brain were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

ABclonal:Immunofluorescence - Phospho-Tau-S262 Rabbit pAb (AP0397)

Immunofluorescence analysis of SH-SY5Y cells using Phospho-Tau-S262 Rabbit pAb(AP0397) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Tau-S262 Rabbit pAb (AP0397)

Immunofluorescence analysis of paraffin-embedded Mouse brain using Phospho-Tau-S262 Rabbit pAb(AP0397) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

You may also interested in:

Overview

Product name Phospho-Tau-S262 Rabbit pAb
Catalog No. AP0397
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S262 of Tau (NP_058518.1).
Sequence VTSKC
Gene ID 4137
Swiss prot P10636
Synonyms TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6; Phospho-Tau-S262
Calculated MW 79kDa
Observed MW

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse brain
Cellular location Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, axon, cytoskeleton, cytosol
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-Tau-S262 Rabbit pAb images

ABclonal:Western blot - Phospho-Tau-S262 Rabbit pAb (AP0397)}

Western blot - Phospho-Tau-S262 Rabbit pAb (AP0397)

Western blot analysis of lysates from Mouse brain, using Phospho-Tau-S262 Rabbit pAb (AP0397) at 1:500 dilution.Mouse brain were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.
ABclonal:Immunofluorescence - Phospho-Tau-S262 Rabbit pAb (AP0397)}

Immunofluorescence - Phospho-Tau-S262 Rabbit pAb (AP0397)

Immunofluorescence analysis of SH-SY5Y cells using Phospho-Tau-S262 Rabbit pAb(AP0397) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Tau-S262 Rabbit pAb (AP0397)}

Immunofluorescence - Phospho-Tau-S262 Rabbit pAb (AP0397)

Immunofluorescence analysis of paraffin-embedded Mouse brain using Phospho-Tau-S262 Rabbit pAb(AP0397) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Inquire About This Product

Submit your question about AP0397 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on MAPT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPT. (Distance between topics and target gene indicate popularity.) MAPT

* Data provided by citexs.com, for reference only.

Publishing research using AP0397? Please let us know so that we can cite the reference in this datasheet.

Antibodies (25)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order