Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-p53-T18 Rabbit pAb (AP0464)

Publications (2) Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-p53-T18 Rabbit pAb (AP0464)

Western blot analysis of lysates from MDA cells, using Phospho-p53-T18 Rabbit pAb (AP0464).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-p53-T18 Rabbit pAb
Catalog No. AP0464
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen A synthetic phosphorylated peptide around T18 of human p53 (NP_000537.3).
Sequence QETFS
Gene ID 7157
Swiss prot P04637
Synonyms P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-T18
Calculated MW 44kDa
Observed MW 54kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples MDA
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

WB (Homo sapiens)

Research Area

Phospho-p53-T18 Rabbit pAb images

ABclonal:Western blot - Phospho-p53-T18 Rabbit pAb (AP0464)}

Western blot - Phospho-p53-T18 Rabbit pAb (AP0464)

Western blot analysis of lysates from MDA cells, using Phospho-p53-T18 Rabbit pAb (AP0464).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53

* Data provided by citexs.com, for reference only.

Publishing research using AP0464? Please let us know so that we can cite the reference in this datasheet.

Antibodies (31)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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