Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (AP0501)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (AP0501)

Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 antibody (AP0501) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb
Catalog No. AP0501
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y352 of human Syk (NP_003168.2).
Sequence SPYAD
Gene ID 68507535
Swiss prot P43405P43403
Synonyms SYK/ZAP70; Phospho-Syk-Y352/ZAP70-Y319
Calculated MW 69kDa/72kDa/35kDa/55kDa
Observed MW 69kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat, Jurkat+Serum
Cellular location cytoplasm, cytosol, early phagosome, nucleus, plasma membrane
Customer validation

WB (Mus musculus)

Research Area

Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb images

ABclonal:Western blot - Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (AP0501)}

Western blot - Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (AP0501)

Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 antibody (AP0501) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on SYK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SYK. (Distance between topics and target gene indicate popularity.) SYK

* Data provided by citexs.com, for reference only.

Publishing research using AP0501? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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