Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-Stat3-Y705 pAb (AP0070)

Publications (2) Datasheet

ABclonal:Western blot - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)
ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)

Overview

Product namePhospho-Stat3-Y705 pAb
Catalog No.AP0070
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic phosphorylated peptide around Y705 of human Stat3 (NP_644805.1).
SequenceAPYLK
Gene ID6774
Swiss protP40763
SynonymsSTAT3; ADMIO; ADMIO1; APRF; HIES; signal transducer and activator of transcription 3
Calculated MW83kDa/87kDa/88kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
Customer validation

IF(Mouse)

WB(Human)

ABclonal:Western blot - Phospho-Stat3-Y705 pAb (AP0070) }

Western blot - Phospho-Stat3-Y705 pAb (AP0070)

Western blot analysis of extracts of HeLa cells, using Phospho-Stat3-Y705 antibody (AP0070) at 1:2000 dilution or STAT3 antibody (A1192). HeLa cells were treated by IFN-α (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat liver using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded rat heart using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human tonsil using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human lung using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human liver using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human appendix using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human placenta using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse liver using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070) }

Immunohistochemistry - Phospho-Stat3-Y705 pAb (AP0070)

Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070) }

Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)

Immunofluorescence analysis of rat brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070) }

Immunofluorescence - Phospho-Stat3-Y705 pAb (AP0070)

Immunofluorescence analysis of mouse brain using Phospho-Stat3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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