Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Src-Y529 Rabbit pAb (AP0185)

Review (1) Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Src-Y529 Rabbit pAb (AP0185)

Western blot analysis of extracts of A-431 cells, using Phospho-Src-Y529 antibody (AP0185) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Review
ABclonal: review for Phospho-Src-Y529 Rabbit pAb(AP0185)

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Overview

Product name Phospho-Src-Y529 Rabbit pAb
Catalog No. AP0185
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y529 of human Src (NP_005408.1).
Sequence EPQYQ
Gene ID 6714
Swiss prot P12931
Synonyms ASV; SRC1; THC6; c-SRC; p60-Src; Phospho-Src-Y529
Calculated MW 60kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples A-431
Cellular location Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton
Customer validation

WB (Human lung cancer cellHomo sapiens, Homo sapiens, Mus musculus, Rattus norvegicus)

IHC (Mus musculus)

IF (Mus musculus, Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-Src-Y529 Rabbit pAb images

ABclonal:Western blot - Phospho-Src-Y529 Rabbit pAb (AP0185)}

Western blot - Phospho-Src-Y529 Rabbit pAb (AP0185)

Western blot analysis of extracts of A-431 cells, using Phospho-Src-Y529 antibody (AP0185) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SRC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SRC. (Distance between topics and target gene indicate popularity.) SRC

* Data provided by citexs.com, for reference only.

Publishing research using AP0185? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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