Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Publications (32) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded mouse kidney using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded rat ovary using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded human colon carcinoma using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded rat lung using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded human breast cancer using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-Smad3-S423/S425 Rabbit mAb
Catalog No. AP0727
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0170

Background

The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S423 & S425 of human Smad3 (P84022).
Sequence SSVS
Gene ID 4088
Swiss prot P84022
Synonyms LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; Phospho-Smad3-S423/S425
Calculated MW 48kDa
Observed MW 55kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P HumanMouseRat
WB MouseRat
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples C6+PMA/TPA
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens, Rattus norvegicus, Mus musculus)

IHC (Mus musculus, Rattus norvegicus, Homo sapiens)

IB (Homo sapiens)

IF (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-Smad3-S423/S425 Rabbit mAb images

ABclonal:Western blot - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)}

Western blot - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)}

Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded mouse kidney using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)}

Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded rat ovary using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)}

Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded human colon carcinoma using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)}

Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded rat lung using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)}

Immunohistochemistry - Phospho-Smad3-S423/S425 Rabbit mAb (AP0727)

Immunohistochemistry analysis of Phospho-Smad3-S423/S425 in paraffin-embedded human breast cancer using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on SMAD3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SMAD3. (Distance between topics and target gene indicate popularity.) SMAD3

* Data provided by citexs.com, for reference only.

Publishing research using AP0727? Please let us know so that we can cite the reference in this datasheet.

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