Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Smad2-S465/S467 Rabbit pAb (AP0925)

Review (1) Publications (5) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-Smad2-S465/S467 Rabbit pAb (AP0925)

Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S465/S467 Rabbit pAb (AP0925) at 1:1000 dilution.HeLa cells were treated by TGF-β (10ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for Phospho-Smad2-S465/S467 Rabbit pAb(AP0925)

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Overview

Product name Phospho-Smad2-S465/S467 Rabbit pAb
Catalog No. AP0925
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S465 & S467 of human Smad2 (NP_005892.1).
Sequence SSMS
Gene ID 4087
Swiss prot Q15796
Synonyms JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; Phospho-Smad2-S465/S467
Calculated MW 52kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Cytoplasm, Nucleus
Customer validation

(A549 (#5) HOP-1 (KK-22) / (#6) TH1-5 (Ac-GF-22-NH2) for 0-100 μM + TGF-β 100 pM for 0.5 hr)

WB (Mus musculus, Homo sapiens)

Research Area

Phospho-Smad2-S465/S467 Rabbit pAb images

ABclonal:Western blot - Phospho-Smad2-S465/S467 Rabbit pAb (AP0925)}

Western blot - Phospho-Smad2-S465/S467 Rabbit pAb (AP0925)

Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S465/S467 Rabbit pAb (AP0925) at 1:1000 dilution.HeLa cells were treated by TGF-β (10ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SMAD2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SMAD2. (Distance between topics and target gene indicate popularity.) SMAD2

* Data provided by citexs.com, for reference only.

Publishing research using AP0925? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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