Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Publications (14) Datasheet

ABclonal:Western blot - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-Y705 antibody (AP0070) at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunohistochemistry of paraffin-embedded Rat brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunohistochemistry of paraffin-embedded Human liver cancer using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunohistochemistry of paraffin-embedded Mouse liver using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunofluorescence analysis of rat brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunofluorescence analysis of mouse brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePhospho-STAT3-Y705 Rabbit pAb
Catalog No.AP0070
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic phosphorylated peptide around Y705 of human Phospho-STAT3-Y705 (NP_644805.1).
SequenceAPYLK
Gene ID6774
Swiss protP40763
SynonymsADMIO; ADMIO1; APRF; HIES; STAT3; Stat3
Calculated MW83kDa/87kDa/88kDa
Observed MW79KDa/86KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - Phospho-STAT3-Y705 Rabbit pAb (AP0070) }

Western blot - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-Y705 antibody (AP0070) at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070) }

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunohistochemistry of paraffin-embedded Rat brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070) }

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunohistochemistry of paraffin-embedded Human liver cancer using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070) }

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunohistochemistry of paraffin-embedded Mouse liver using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Phospho-STAT3-Y705 Rabbit pAb (AP0070) }

Immunofluorescence - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunofluorescence analysis of rat brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-STAT3-Y705 Rabbit pAb (AP0070) }

Immunofluorescence - Phospho-STAT3-Y705 Rabbit pAb (AP0070)

Immunofluorescence analysis of mouse brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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