Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-STAT2-Y690 Rabbit pAb (AP0284)

Publications (7) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-STAT2-Y690 Rabbit pAb (AP0284)

Western blot analysis of various lysates, using Phospho-STAT2-Y690 Rabbit pAb (AP0284) at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name Phospho-STAT2-Y690 Rabbit pAb
Catalog No. AP0284
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. The protein mediates innate antiviral activity. Mutations in this gene result in Immunodeficiency 44.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y690 of human STAT2 (NP_005410.1).
Sequence RRKYLK
Gene ID 6773
Swiss prot P52630
Synonyms P113; IMD44; ISGF-3; PTORCH3; STAT113; Phospho-STAT2-Y690
Calculated MW 98kDa
Observed MW 97kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-STAT2-Y690 Rabbit pAb images

ABclonal:Western blot - Phospho-STAT2-Y690 Rabbit pAb (AP0284)}

Western blot - Phospho-STAT2-Y690 Rabbit pAb (AP0284)

Western blot analysis of various lysates, using Phospho-STAT2-Y690 Rabbit pAb (AP0284) at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on STAT2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to STAT2. (Distance between topics and target gene indicate popularity.) STAT2

* Data provided by citexs.com, for reference only.

Publishing research using AP0284? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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