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Phospho-STAT1-Y701 pAb (AP0135)

Review (1)Publications (2) Datasheet

ABclonal:Western blot - Phospho-STAT1-Y701 pAb (AP0135)
Review
ABclonal:Western blot (WB) review for Phospho-STAT1-Y701 pAb(AP0135)

Overview

Product namePhospho-STAT1-Y701 pAb
Catalog No.AP0135
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y701 of human STAT1
SequenceEmail for sequence
Gene ID6772
Swiss protP42224
SynonymsSTAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; signal transducer and activator of transcription 1
Calculated MW83kDa/87kDa
Observed MW85kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human chronic myeloid leukemia cells, Mouse)

ABclonal:Western blot - Phospho-STAT1-Y701 pAb (AP0135) }

Western blot - Phospho-STAT1-Y701 pAb (AP0135)

Western blot analysis of extracts of HeLa cells, using Phospho-STAT1-Y701 antibody (AP0135) at 1:1000 dilution or STAT1 antibody (A12075). HeLa cells were treated by IFN-α (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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