Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-STAT1-Y701 Rabbit pAb (AP0135)

Review (1) Publications (9) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-STAT1-Y701 Rabbit pAb (AP0135)

Western blot analysis of lysates from Hela cells, using Phospho-STAT1-Y701 (AP0135) at 1:630 dilution.HeLa cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Phospho-STAT1-Y701 Rabbit pAb (AP0135)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT1-Y701 (AP0135) at 1:630 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review
ABclonal: review for Phospho-STAT1-Y701 Rabbit pAb(AP0135)

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Overview

Product name Phospho-STAT1-Y701 Rabbit pAb
Catalog No. AP0135
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y701 of human STAT1 (NP_009330.1).
Sequence TGYIK
Gene ID 6772
Swiss prot P42224
Synonyms CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; Phospho-STAT1-Y701
Calculated MW 87kDa
Observed MW 84kDa/91kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Human chronic myeloid leukemia cellsMus musculus, Mus musculus, Homo sapiens)

IP (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-STAT1-Y701 Rabbit pAb images

ABclonal:Western blot - Phospho-STAT1-Y701 Rabbit pAb (AP0135)}

Western blot - Phospho-STAT1-Y701 Rabbit pAb (AP0135)

Western blot analysis of lysates from Hela cells, using Phospho-STAT1-Y701 (AP0135) at 1:630 dilution.HeLa cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Phospho-STAT1-Y701 Rabbit pAb (AP0135)}

Western blot - Phospho-STAT1-Y701 Rabbit pAb (AP0135)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT1-Y701 (AP0135) at 1:630 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on STAT1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to STAT1. (Distance between topics and target gene indicate popularity.) STAT1

* Data provided by citexs.com, for reference only.

Publishing research using AP0135? Please let us know so that we can cite the reference in this datasheet.

Antibodies (9)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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