Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-SRC Family-Y416 Rabbit pAb (AP0452)

Reviews (3) Publications (7) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0452)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (AP0452) at 1:1000 dilution.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Review
ABclonal: review for Phospho-SRC Family-Y416 Rabbit pAb(AP0452)
Review
ABclonal: review for Phospho-SRC Family-Y416 Rabbit pAb(AP0452)
Review
ABclonal: review for Phospho-SRC Family-Y416 Rabbit pAb(AP0452)

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Overview

Product name Phospho-SRC Family-Y416 Rabbit pAb
Catalog No. AP0452
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y416 of human SRC Family (NP_005408.1).
Sequence NEYTA
Gene ID 253430553932406767147525
Swiss prot P12931
Synonyms ASV; SRC1; THC6; c-SRC; p60-Src; SRC; Phospho-SRC Family-Y416
Calculated MW 60kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3
Cellular location Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton
Customer validation

WB (Human breast cancer, Human lung cancer cellMus musculus, Homo sapiens, Mus musculus, Rattus norvegicus)

IP (H460 and A549)

IF (Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Phospho-SRC Family-Y416 Rabbit pAb images

ABclonal:Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0452)}

Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0452)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (AP0452) at 1:1000 dilution.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on Src Family. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Src Family. (Distance between topics and target gene indicate popularity.) Src Family

* Data provided by citexs.com, for reference only.

Publishing research using AP0452? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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