Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-SQSTM1/p62-S349 Rabbit pAb (A20737)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-SQSTM1/p62-S349 Rabbit pAb (A20737)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SQSTM1/p62-S349 antibody (A20737) at 1:500 dilution.NIH/3T3 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-SQSTM1/p62-S349 Rabbit pAb (A20737)

Western blot analysis of extracts of various cell lines, using Phospho-SQSTM1/p62-S349 antibody (A20737) at 1:500 dilution.HeLa cells and C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name Phospho-SQSTM1/p62-S349 Rabbit pAb
Catalog No. A20737
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S349 of human SQSTM1/p62 (NP_003891.1).
Sequence DPSTG
Gene ID 8878
Swiss prot Q13501
Synonyms p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3; Phospho-SQSTM1/p62-S349
Calculated MW 48kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, C6, NIH/3T3
Cellular location Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-SQSTM1/p62-S349 Rabbit pAb images

ABclonal:Western blot - Phospho-SQSTM1/p62-S349 Rabbit pAb (A20737)}

Western blot - Phospho-SQSTM1/p62-S349 Rabbit pAb (A20737)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SQSTM1/p62-S349 antibody (A20737) at 1:500 dilution.NIH/3T3 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-SQSTM1/p62-S349 Rabbit pAb (A20737)}

Western blot - Phospho-SQSTM1/p62-S349 Rabbit pAb (A20737)

Western blot analysis of extracts of various cell lines, using Phospho-SQSTM1/p62-S349 antibody (A20737) at 1:500 dilution.HeLa cells and C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SQSTM1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SQSTM1. (Distance between topics and target gene indicate popularity.) SQSTM1

* Data provided by citexs.com, for reference only.

Publishing research using A20737? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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