Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Smad1-S465 Rabbit pAb (AP0268)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Smad1-S465 Rabbit pAb (AP0268)

Western blot analysis of extracts from HeLa cells using Phospho-Smad1-S465 antibody (AP0268).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-Smad1-S465 Rabbit pAb
Catalog No. AP0268
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S465 of human Smad1
Sequence Email for sequence
Gene ID 4086
Swiss prot Q15797
Synonyms BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1; Phospho-Smad1-S465
Calculated MW 52kDa
Observed MW 55kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Cytoplasm, Nucleus

Research Area

Phospho-Smad1-S465 Rabbit pAb images

ABclonal:Western blot - Phospho-Smad1-S465 Rabbit pAb (AP0268)}

Western blot - Phospho-Smad1-S465 Rabbit pAb (AP0268)

Western blot analysis of extracts from HeLa cells using Phospho-Smad1-S465 antibody (AP0268).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on SMAD1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SMAD1. (Distance between topics and target gene indicate popularity.) SMAD1

* Data provided by citexs.com, for reference only.

Publishing research using AP0268? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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