Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-SHC1-Y427 Rabbit pAb (AP0266)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-SHC1-Y427 Rabbit pAb (AP0266)

Western blot analysis of extracts from 293 cells using Phospho-SHC1-Y427 antibody (AP0266).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-SHC1-Y427 Rabbit pAb
Catalog No. AP0266
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding Y427 of human SHC1
Sequence Email for sequence
Gene ID 6464
Swiss prot P29353
Synonyms SHC; SHCA; Phospho-SHC1-Y427
Calculated MW 63kDa
Observed MW 50kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293
Cellular location Cytoplasm, Mitochondrion, Mitochondrion matrix

Research Area

Phospho-SHC1-Y427 Rabbit pAb images

ABclonal:Western blot - Phospho-SHC1-Y427 Rabbit pAb (AP0266)}

Western blot - Phospho-SHC1-Y427 Rabbit pAb (AP0266)

Western blot analysis of extracts from 293 cells using Phospho-SHC1-Y427 antibody (AP0266).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on SHC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SHC1. (Distance between topics and target gene indicate popularity.) SHC1

* Data provided by citexs.com, for reference only.

Publishing research using AP0266? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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