Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-RPS6-S240/244 pAb (AP0537)

Datasheet

ABclonal:Western blot - Phospho-RPS6-S240/244 pAb (AP0537)
ABclonal:Immunoprecipitation - Phospho-RPS6-S240/244 pAb (AP0537)

Overview

Product namePhospho-RPS6-S240/244 pAb
Catalog No.AP0537
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenA synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_001001.2).
SequenceSTSKS
Gene ID6194
Swiss protP62753
SynonymsRPS6; S6; ribosomal protein S6
Calculated MW28kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3
Cellular location
ABclonal:Western blot - Phospho-RPS6-S240/244 pAb (AP0537) }

Western blot - Phospho-RPS6-S240/244 pAb (AP0537)

Western blot analysis of extracts of various cell lines, using Phospho-RPS6KB1-S240/244 antibody (AP0537) at 1:1000 dilution or RPS6 antibody (A6058). HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - Phospho-RPS6-S240/244 pAb (AP0537) }

Immunoprecipitation - Phospho-RPS6-S240/244 pAb (AP0537)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-RPS6-S240/244 pAb (AP0537). Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 pAb (AP0537) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.

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