Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-p70 S6 Kinase 1-S424 Rabbit pAb (AP0253)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p70 S6 Kinase 1-S424 Rabbit pAb (AP0253)

Western blot analysis of extracts from MCF cells using Phospho-p70 S6 Kinase 1-S424 antibody (AP0253).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-p70 S6 Kinase 1-S424 Rabbit pAb
Catalog No. AP0253
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S424 of human P70 S6K
Sequence Email for sequence
Gene ID 6198
Swiss prot P23443
Synonyms S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha; Phospho-p70 S6 Kinase 1-S424
Calculated MW 59kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples MCF7
Cellular location Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome
Customer validation

WB (Mus musculus)

Research Area

Phospho-p70 S6 Kinase 1-S424 Rabbit pAb images

ABclonal:Western blot - Phospho-p70 S6 Kinase 1-S424 Rabbit pAb (AP0253)}

Western blot - Phospho-p70 S6 Kinase 1-S424 Rabbit pAb (AP0253)

Western blot analysis of extracts from MCF cells using Phospho-p70 S6 Kinase 1-S424 antibody (AP0253).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on RPS6KB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RPS6KB1. (Distance between topics and target gene indicate popularity.) RPS6KB1

* Data provided by citexs.com, for reference only.

Publishing research using AP0253? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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