Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-RIPK1-S166 Rabbit pAb (AP1115)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-RIPK1-S166 Rabbit pAb (AP1115)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RIPK1-S166 Rabbit pAb (AP1115) at 1:1000 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name Phospho-RIPK1-S166 Rabbit pAb
Catalog No. AP1115
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables protein homodimerization activity and protein serine/threonine kinase activity. Involved in several processes, including necroptotic signaling pathway; negative regulation of cardiac muscle cell proliferation; and regulation of signal transduction. Acts upstream of or within several processes, including positive regulation of I-kappaB kinase/NF-kappaB signaling; regulation of extrinsic apoptotic signaling pathway; and ripoptosome assembly involved in necroptotic process. Predicted to be located in several cellular components, including cytosol; membrane raft; and mitochondrion. Predicted to be part of death-inducing signaling complex; receptor complex; and ripoptosome. Is expressed in several structures, including central nervous system; heart; liver; lung; and tail. Human ortholog(s) of this gene implicated in immunodeficiency 57. Orthologous to human RIPK1 (receptor interacting serine/threonine kinase 1).

Immunogen information

Immunogen A synthetic phosphorylated peptide around S166 of mouse RIPK1 (NP_033094.3).
Sequence TWSKL
Gene ID 19766
Swiss prot Q60855
Synonyms RIP; Rinp; Rip1; RIP-1; D330015H01Rik; Phospho-RIPK1-S166
Calculated MW 75kDa
Observed MW 82kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3
Cellular location mitochondrion, ripoptosome
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Phospho-RIPK1-S166 Rabbit pAb images

ABclonal:Western blot - Phospho-RIPK1-S166 Rabbit pAb (AP1115)}

Western blot - Phospho-RIPK1-S166 Rabbit pAb (AP1115)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RIPK1-S166 Rabbit pAb (AP1115) at 1:1000 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on Ripk1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Ripk1. (Distance between topics and target gene indicate popularity.) Ripk1

* Data provided by citexs.com, for reference only.

Publishing research using AP1115? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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