Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-RIPK1-S166 Rabbit pAb (AP1115)

Datasheet

ABclonal:Western blot - Phospho-RIPK1-S166 Rabbit pAb (AP1115)

Overview

Product namePhospho-RIPK1-S166 Rabbit pAb
Catalog No.AP1115
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Serine-threonine kinase which is a key regulator of both cell death and cell survival (PubMed:25459879). Exhibits kinase activity-dependent functions that trigger cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival (PubMed:31519887, PubMed:31519886). Initiates ripoptocide which describes cell death that is dependent on RIPK1, be it apoptosis or necroptosis (PubMed:31457011). Upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kB pathway (PubMed:31519887, PubMed:31519886). Specific conditions can however activate RIPK1, and its kinase activity then regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8) and the complex IIb (RIPK1-RIPK3-MLKL) and these complexes respectively drive apoptosis or necroptosis, a regulated form of necrosis (PubMed:29440439, PubMed:30988283, PubMed:31519887, PubMed:31519886). During embryonic development suppresses apoptosis and necroptosis and prevents the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8 (PubMed:30867408, PubMed:30185824, PubMed:31511692). Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (By similarity). Required for ZBP1-induced NF-kappaB activation and activation of NF-kappaB by DNA damage and IR (PubMed:12654725, PubMed:19590578).
ImmunogenA phospho specific peptide corresponding to residues surrounding S166 of Mouse RIPK1.
SequenceEmail for sequence
Gene ID19766
Swiss protQ60855
SynonymsD330015H01Rik; RIP; Rinp; Rip1
Calculated MW
Observed MW82kDa
ReactivityHuman, Mouse,
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesNIH/3T3
Cellular location
    ABclonal:Western blot - Phospho-RIPK1-S166 Rabbit pAb (AP1115) }

    Western blot - Phospho-RIPK1-S166 Rabbit pAb (AP1115)

    Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RIPK1-S166 antibody (AP1115) at 1:1000 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    Inquire About This Product

    Submit your question about AP1115 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Select size and quantity
    Contact us to order