Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-NF-kB p65/RelA-T505 Rabbit pAb (AP0190)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-NF-kB p65/RelA-T505 Rabbit pAb (AP0190)

Western blot analysis of extracts from HL60 cells untreated or treated with TNF-a, using Phospho-NF-kB p65/RelA-T505 antibody (AP0190).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-NF-kB p65/RelA-T505 Rabbit pAb
Catalog No. AP0190
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T505 of human RelA
Sequence Email for sequence
Gene ID 5970
Swiss prot Q04206
Synonyms p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-T505
Calculated MW 60kDa
Observed MW 60kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HL60
Cellular location Cytoplasm, Nucleus

Research Area

Phospho-NF-kB p65/RelA-T505 Rabbit pAb images

ABclonal:Western blot - Phospho-NF-kB p65/RelA-T505 Rabbit pAb (AP0190)}

Western blot - Phospho-NF-kB p65/RelA-T505 Rabbit pAb (AP0190)

Western blot analysis of extracts from HL60 cells untreated or treated with TNF-a, using Phospho-NF-kB p65/RelA-T505 antibody (AP0190).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on RELA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RELA. (Distance between topics and target gene indicate popularity.) RELA

* Data provided by citexs.com, for reference only.

Publishing research using AP0190? Please let us know so that we can cite the reference in this datasheet.

Antibodies (23)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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