Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-RELA-S311 pAb (AP0445)

Datasheet

ABclonal:Western blot - Phospho-RELA-S311 pAb (AP0445)
ABclonal:Immunohistochemistry - Phospho-RELA-S311 pAb (AP0445)

Overview

Product namePhospho-RELA-S311 pAb
Catalog No.AP0445
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S311 of human RELA (NP_068810.3).
SequenceFKSIM
Gene ID5970
Swiss protQ04206
SynonymsRELA; NFKB3; p65; transcription factor p65
Calculated MW58kDa/59kDa/60kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - Phospho-RELA-S311 pAb (AP0445) }

Western blot - Phospho-RELA-S311 pAb (AP0445)

Western blot analysis of extracts of HeLa cells, using Phospho-RELA-S311 antibody (AP0445) at 1:1000 dilution or RELA antibody (A11201). HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-RELA-S311 pAb (AP0445) }

Immunohistochemistry - Phospho-RELA-S311 pAb (AP0445)

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-RELA-S311 antibody (AP0445) at dilution of 1:100 (40x lens).

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