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Phospho-NF-kB p65-S311 pAb (AP0445)


ABclonal:Western blot - Phospho-NF-kB p65-S311 pAb (AP0445)
ABclonal:Immunohistochemistry - Phospho-NF-kB p65-S311 pAb (AP0445)


Product namePhospho-NF-kB p65-S311 pAb
Catalog No.AP0445
Host speciesRabbit
Purification methodAffinity purification
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S311 of human NF-kB p65 (NP_068810.3).
Gene ID5970
Swiss protQ04206
SynonymsRELA; NFKB3; p65; transcription factor p65
Calculated MW58kDa/59kDa/60kDa
Observed MW60kDa
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - Phospho-NF-kB p65-S311 pAb (AP0445) }

Western blot - Phospho-NF-kB p65-S311 pAb (AP0445)

Western blot analysis of extracts of HeLa cells, using Phospho-NF-kB p65-S311 antibody (AP0445) at 1:1000 dilution or RELA antibody (A11201). HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65-S311 pAb (AP0445) }

Immunohistochemistry - Phospho-NF-kB p65-S311 pAb (AP0445)

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-NF-kB p65-S311 antibody (AP0445) at dilution of 1:100 (40x lens).

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