Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Rac1/2/3-S71 Rabbit pAb (AP0442)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Rac1/2/3-S71 Rabbit pAb (AP0442)

Western blot analysis of extracts from 293 cells, using Phospho-Rac1/2/3-S71 antibody (AP0442).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-Rac1/2/3-S71 Rabbit pAb
Catalog No. AP0442
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S71 of human RAC1
Sequence Email for sequence
Gene ID 5879
Swiss prot P63000
Synonyms MIG5; MRD48; Rac-1; TC-25; p21-Rac1; Phospho-Rac1/2/3-S71
Calculated MW 21kDa
Observed MW 30kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293
Cellular location Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor, Melanosome

Research Area

Phospho-Rac1/2/3-S71 Rabbit pAb images

ABclonal:Western blot - Phospho-Rac1/2/3-S71 Rabbit pAb (AP0442)}

Western blot - Phospho-Rac1/2/3-S71 Rabbit pAb (AP0442)

Western blot analysis of extracts from 293 cells, using Phospho-Rac1/2/3-S71 antibody (AP0442).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on RAC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RAC1. (Distance between topics and target gene indicate popularity.) RAC1

* Data provided by citexs.com, for reference only.

Publishing research using AP0442? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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