Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-FAK-Y576/577 Rabbit pAb (AP0536)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-FAK-Y576/577 Rabbit pAb (AP0536)

Western blot analysis of extracts of Jurkat and Raji cells, using Phospho-FAK-Y576/577 antibody (AP0536) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 μg/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes.Raji cells were treated by PMA/TPA (200nM) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name Phospho-FAK-Y576/577 Rabbit pAb
Catalog No. AP0536
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y576 & Y577 of human PTK2 (NP_722560.1).
Sequence STYYKA
Gene ID 5747
Swiss prot Q05397
Synonyms FAK; FADK; FAK1; FRNK; FADK 1; PPP1R71; p125FAK; pp125FAK; Phospho-FAK-Y576/577
Calculated MW 119kDa
Observed MW 130kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat, Raji
Cellular location Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cell cortex, centrosome, cytoskeleton, focal adhesion, microtubule organizing center

Research Area

Phospho-FAK-Y576/577 Rabbit pAb images

ABclonal:Western blot - Phospho-FAK-Y576/577 Rabbit pAb (AP0536)}

Western blot - Phospho-FAK-Y576/577 Rabbit pAb (AP0536)

Western blot analysis of extracts of Jurkat and Raji cells, using Phospho-FAK-Y576/577 antibody (AP0536) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 μg/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes.Raji cells were treated by PMA/TPA (200nM) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PTK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PTK2. (Distance between topics and target gene indicate popularity.) PTK2

* Data provided by citexs.com, for reference only.

Publishing research using AP0536? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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