Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PTK2B-Y402 Rabbit pAb (AP0214)

Review (1) Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PTK2B-Y402 Rabbit pAb (AP0214)

Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit pAb (AP0214) at 1:1000 dilution.Jurkat cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review
ABclonal: review for Phospho-PTK2B-Y402 Rabbit pAb(AP0214)

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Overview

Product name Phospho-PTK2B-Y402 Rabbit pAb
Catalog No. AP0214
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y402 of human PTK2B (NP_004094.3).
Sequence DIYAE
Gene ID 2185
Swiss prot Q14289
Synonyms PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK; Phospho-PTK2B-Y402
Calculated MW 116kDa
Observed MW 116kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Jurkat
Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cell cortex, focal adhesion, lamellipodium, perinuclear region
Customer validation

WB (Human lung cancer cellHomo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-PTK2B-Y402 Rabbit pAb images

ABclonal:Western blot - Phospho-PTK2B-Y402 Rabbit pAb (AP0214)}

Western blot - Phospho-PTK2B-Y402 Rabbit pAb (AP0214)

Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit pAb (AP0214) at 1:1000 dilution.Jurkat cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PTK2B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PTK2B. (Distance between topics and target gene indicate popularity.) PTK2B

* Data provided by citexs.com, for reference only.

Publishing research using AP0214? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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