Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PKC zeta-T410 Rabbit pAb (AP0433)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PKC zeta-T410 Rabbit pAb (AP0433)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PRKCA were treated by TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight, using Phospho-PRKCA-T410 Rabbit pAb (AP0433) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name Phospho-PKC zeta-T410 Rabbit pAb
Catalog No. AP0433
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T410 of human PKC (pan) (zeta).
Sequence Email for sequence
Gene ID 5590
Swiss prot Q05513
Synonyms PKC2; PKC-ZETA; Phospho-PKC zeta-T410
Calculated MW 68kDa
Observed MW 85kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293F transfected with PKC zeta treated by TPA
Cellular location Cell junction, Cytoplasm, Endosome

Documents

Certificate of Compliance

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Research Area

Phospho-PKC zeta-T410 Rabbit pAb images

ABclonal:Western blot - Phospho-PKC zeta-T410 Rabbit pAb (AP0433)}

Western blot - Phospho-PKC zeta-T410 Rabbit pAb (AP0433)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PRKCA were treated by TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight, using Phospho-PRKCA-T410 Rabbit pAb (AP0433) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKCZ. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKCZ. (Distance between topics and target gene indicate popularity.) PRKCZ

* Data provided by citexs.com, for reference only.

Publishing research using AP0433? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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