Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PRKCQ-T538 Rabbit pAb (AP0798)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-PRKCQ-T538 Rabbit pAb (AP0798)

Western blot analysis of lysates from Jurkat and HeLa cells, using Phospho-PRKCQ-T538 Rabbit pAb (AP0798) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name Phospho-PRKCQ-T538 Rabbit pAb
Catalog No. AP0798
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T538 of human PRKCQ (NP_006248.1).
Sequence KTNTFC
Gene ID 5588
Swiss prot Q04759
Synonyms PRKCT; nPKC-theta; Phospho-PRKCQ-T538
Calculated MW 82kDa
Observed MW 98kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat, HeLa
Cellular location Cell membrane, Cytoplasm, Peripheral membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-PRKCQ-T538 Rabbit pAb images

ABclonal:Western blot - Phospho-PRKCQ-T538 Rabbit pAb (AP0798)}

Western blot - Phospho-PRKCQ-T538 Rabbit pAb (AP0798)

Western blot analysis of lysates from Jurkat and HeLa cells, using Phospho-PRKCQ-T538 Rabbit pAb (AP0798) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKCQ. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKCQ. (Distance between topics and target gene indicate popularity.) PRKCQ

* Data provided by citexs.com, for reference only.

Publishing research using AP0798? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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