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Phospho-PPP1R12A-S507 pAb (AP0769)


ABclonal:Western blot - Phospho-PPP1R12A-S507 pAb (AP0769)


Product namePhospho-PPP1R12A-S507 pAb
Catalog No.AP0769
Host speciesRabbit
Purification methodAffinity purification
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S507 of human PPP1R12A (NP_001137357.1).
Gene ID4659
Swiss protO14974
SynonymsPPP1R12A; M130; MBS; MYPT1; protein phosphatase 1 regulatory subunit 12A
Calculated MW105kDa/109kDa/111kDa/115kDa
Observed MW135kDa
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, Jurkat
Cellular locationCytoplasm
ABclonal:Western blot - Phospho-PPP1R12A-S507 pAb (AP0769) }

Western blot - Phospho-PPP1R12A-S507 pAb (AP0769)

Western blot analysis of extracts of various cell lines, using Phospho-PPP1R12A-S507 antibody (AP0769) at 1:2000 dilution or PPP1R12A antibody (A0587). HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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