Western blot - Phospho-PPP1R12A-S507 pAb (AP0769)
Western blot analysis of extracts of various cell lines, using Phospho-PPP1R12A-S507 antibody (AP0769) at 1:2000 dilution or PPP1R12A antibody (A0587). HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.