Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PPP1R12A-S507 Rabbit pAb (AP0769)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-PPP1R12A-S507 Rabbit pAb (AP0769)

Western blot analysis of various lysates using Phospho-PPP1R12A-S507 Rabbit pAb (AP0769) at 1:2000 dilution or PPP1R12A antibody (A0587). HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name Phospho-PPP1R12A-S507 Rabbit pAb
Catalog No. AP0769
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S507 of human PPP1R12A (NP_001137357.1).
Sequence LASTS
Gene ID 4659
Swiss prot O14974
Synonyms MBS; GUBS; M130; MYPT1; Phospho-PPP1R12A-S507
Calculated MW 115kDa
Observed MW 135kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, Jurkat
Cellular location Cytoplasm

Documents

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Research Area

Phospho-PPP1R12A-S507 Rabbit pAb images

ABclonal:Western blot - Phospho-PPP1R12A-S507 Rabbit pAb (AP0769)}

Western blot - Phospho-PPP1R12A-S507 Rabbit pAb (AP0769)

Western blot analysis of various lysates using Phospho-PPP1R12A-S507 Rabbit pAb (AP0769) at 1:2000 dilution or PPP1R12A antibody (A0587). HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PPP1R12A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PPP1R12A. (Distance between topics and target gene indicate popularity.) PPP1R12A

* Data provided by citexs.com, for reference only.

Publishing research using AP0769? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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