Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 60s.

ABclonal:Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Western blot analysis of lysates from HeLa cells using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at 1:500 dilution. HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Enhanced Kit (RM00021).
Exposuretime: 30s.

ABclonal:Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Western blot analysis of lysates from C6 cells using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at 1:500 dilution. C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 60s.

ABclonal:Immunohistochemistry - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Immunohistochemistry analysis of Phospho-PPP1CA-T320 in paraffin-embedded rat lung using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Immunohistochemistry analysis of Phospho-PPP1CA-T320 in paraffin-embedded human gastric cancer using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

You may also interested in:

Overview

Product name Phospho-PPP1CA-T320 Rabbit pAb
Catalog No. AP0786
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T320 of human PPP1CA (NP_002699.1).
Sequence PITPP
Gene ID 5499
Swiss prot P62136
Synonyms PP1A; PP-1A; PPP1A; PP1alpha; Phospho-PPP1CA-T320
Calculated MW 38kDa
Observed MW 38kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples C6, NIH/3T3, HeLa
Cellular location Cytoplasm, Nucleus, nucleolus, nucleoplasm
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-PPP1CA-T320 Rabbit pAb images

ABclonal:Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)}

Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 60s.
ABclonal:Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)}

Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Western blot analysis of lysates from HeLa cells using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at 1:500 dilution. HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Enhanced Kit (RM00021).
Exposuretime: 30s.
ABclonal:Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)}

Western blot - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Western blot analysis of lysates from C6 cells using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at 1:500 dilution. C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 60s.
ABclonal:Immunohistochemistry - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)}

Immunohistochemistry - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Immunohistochemistry analysis of Phospho-PPP1CA-T320 in paraffin-embedded rat lung using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)}

Immunohistochemistry - Phospho-PPP1CA-T320 Rabbit pAb (AP0786)

Immunohistochemistry analysis of Phospho-PPP1CA-T320 in paraffin-embedded human gastric cancer using Phospho-PPP1CA-T320 Rabbit pAb (AP0786) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Inquire About This Product

Submit your question about AP0786 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on PPP1CA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PPP1CA. (Distance between topics and target gene indicate popularity.) PPP1CA

* Data provided by citexs.com, for reference only.

Publishing research using AP0786? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order