Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PLN-T17 Rabbit pAb (AP0910)

Publications (5) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PLN-T17 Rabbit pAb (AP0910)

Western blot analysis of HeLa, using Phospho-PLN-T17 Rabbit pAb (AP0910) at 1:400 dilution.HeLa cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-PLN-T17 Rabbit pAb (AP0910)

Immunohistochemistry analysis of paraffin-embedded human appendix using Phospho-phospholamban-T17 antibody (AP0910) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-PLN-T17 Rabbit pAb
Catalog No. AP0910
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T17 of human PLN (NP_002658.1).
Sequence ASTIE
Gene ID 5350
Swiss prot P26678
Synonyms PLB; CMD1P; CMH18; Phospho-PLN-T17
Calculated MW 6kDa
Observed MW 24kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa
Cellular location endoplasmic reticulum, mitochondrial membrane, mitochondrion, perinuclear region of cytoplasm
Customer validation

WB (Rattus norvegicus, Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Research Area

Phospho-PLN-T17 Rabbit pAb images

ABclonal:Western blot - Phospho-PLN-T17 Rabbit pAb (AP0910)}

Western blot - Phospho-PLN-T17 Rabbit pAb (AP0910)

Western blot analysis of HeLa, using Phospho-PLN-T17 Rabbit pAb (AP0910) at 1:400 dilution.HeLa cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-PLN-T17 Rabbit pAb (AP0910)}

Immunohistochemistry - Phospho-PLN-T17 Rabbit pAb (AP0910)

Immunohistochemistry analysis of paraffin-embedded human appendix using Phospho-phospholamban-T17 antibody (AP0910) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on PLN. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PLN. (Distance between topics and target gene indicate popularity.) PLN

* Data provided by citexs.com, for reference only.

Publishing research using AP0910? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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