Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PLK1-T210 Rabbit pAb (AP0519)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

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Overview

Product name Phospho-PLK1-T210 Rabbit pAb
Catalog No. AP0519
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T210 of human PLK1 (NP_005021.2).
Sequence KKTLC
Gene ID 5347
Swiss prot P53350
Synonyms PLK; STPK13; Phospho-PLK1-T210
Calculated MW 68kDa
Observed MW Refer to figures

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples
Cellular location Chromosome, Cytoplasm, Midbody, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle
Customer validation

WB (Homo sapiens)

Research Area

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Related Targets

Based on existing publications, the following genes are closely related to research on PLK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PLK1. (Distance between topics and target gene indicate popularity.) PLK1

* Data provided by citexs.com, for reference only.

Publishing research using AP0519? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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