Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785)

Western blot analysis of Raji, using Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785) at 1:2000 dilution.Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb
Catalog No. AP0785
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y759 of human PLC gamma 2 (PLCG2) (NP_002652.2).
Sequence RMYVD
Gene ID 5336
Swiss prot P16885
Synonyms FCAS3; APLAID; PLC-IV; PLC-gamma-2; Phospho-PLC gamma 2 (PLCG2)-Y759
Calculated MW 148kDa
Observed MW 150kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Raji
Cellular location cytoplasm, cytosol, extracellular exosome, perinuclear region of cytoplasm, plasma membrane, ruffle membrane
Customer validation

WB (Homo sapiens)

IF (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb images

ABclonal:Western blot - Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785)}

Western blot - Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785)

Western blot analysis of Raji, using Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785) at 1:2000 dilution.Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PLCG2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PLCG2. (Distance between topics and target gene indicate popularity.) PLCG2

* Data provided by citexs.com, for reference only.

Publishing research using AP0785? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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