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Phospho-PLCG2-Y759 pAb (AP0785)

Datasheet

ABclonal:Western blot - Phospho-PLCG2-Y759 pAb (AP0785)

Overview

Product namePhospho-PLCG2-Y759 pAb
Catalog No.AP0785
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
ImmunogenA synthetic phosphorylated peptide around Y759 of human PLCG2 (NP_002652.2).
SequenceRMYVD
Gene ID5336
Swiss protP16885
SynonymsPLCG2; APLAID; FCAS3; PLC-IV; PLC-gamma-2; phospholipase C gamma 2
Calculated MW147kDa
Observed MW150kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRaji
Cellular location
ABclonal:Western blot - Phospho-PLCG2-Y759 pAb (AP0785) }

Western blot - Phospho-PLCG2-Y759 pAb (AP0785)

Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y759 antibody (AP0785) at 1:2000 dilution or PLCG2 antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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