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Phospho-PLCG1-S1248 pAb (AP0827)

Datasheet

ABclonal:Western blot - Phospho-PLCG1-S1248 pAb (AP0827)
ABclonal:Immunohistochemistry - Phospho-PLCG1-S1248 pAb (AP0827)

Overview

Product namePhospho-PLCG1-S1248 pAb
Catalog No.AP0827
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes the formation of inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S1248 of human PLCG1 (NP_002651.2).
SequenceREGSF
Gene ID5335
Swiss protP19174
SynonymsPLCG1; NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1; phospholipase C gamma 1
Calculated MW148kDa
Observed MW149kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat
Cellular locationCell projection, lamellipodium, ruffle
ABclonal:Western blot - Phospho-PLCG1-S1248 pAb (AP0827) }

Western blot - Phospho-PLCG1-S1248 pAb (AP0827)

Western blot analysis of extracts of Jurkat cells, using Phospho-PLCG1-S1248 antibody (AP0827) at dilution or PLCG1 antibody (A15704). Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-PLCG1-S1248 pAb (AP0827) }

Immunohistochemistry - Phospho-PLCG1-S1248 pAb (AP0827)

Immunohistochemistry of paraffin-embedded human placenta using Phospho-PLCG1-S1248 antibody (AP0827) at dilution of 1:100 (40x lens).

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