Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 pAb (AP0191) at 1:1000 dilution or PRKCA antibody (A13342).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Immunohistochemistry analysis of Phospho-PKCalpha/beta II-T638/641 in paraffin-embedded human lung carcinoma tissue, using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191).

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Overview

Product name Phospho-PKCalpha/beta II-T638/641 Rabbit pAb
Catalog No. AP0191
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T638 of human PKCalpha/beta IIPRKCA (NP_002728.2).
Sequence VLTPP
Gene ID 55785579
Swiss prot P17252P05771
Synonyms PKC-beta; PKCB; PRKCB1; PRKCB2; Phospho-PKCalpha/beta II-T638/641
Calculated MW 76kDa/77kDa
Observed MW 80kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples NIH/3T3
Cellular location Cytoplasm, Membrane, Nucleus, Peripheral membrane protein

Documents

Certificate of Compliance

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Lot number

Phospho-PKCalpha/beta II-T638/641 Rabbit pAb images

ABclonal:Western blot - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)}

Western blot - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 pAb (AP0191) at 1:1000 dilution or PRKCA antibody (A13342).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)}

Immunohistochemistry - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Immunohistochemistry analysis of Phospho-PKCalpha/beta II-T638/641 in paraffin-embedded human lung carcinoma tissue, using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)}

Immunofluorescence - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191).

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Related Targets

Based on existing publications, the following genes are closely related to research on PRKCA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKCA. (Distance between topics and target gene indicate popularity.) PRKCA

* Data provided by citexs.com, for reference only.

Publishing research using AP0191? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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