Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Publications (32) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis of extracts of HeLa cells, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 antibody (AP0854) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product namePhospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb
Catalog No.AP0854
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Immunogen information

ImmunogenA synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1).
SequenceRLYEE
Gene ID529552968503
Swiss protP27986O00459Q92569
SynonymsPIK3R1/PIK3R2/PIK3R3
Calculated MW42-54kDa/81-84kDa
Observed MW85kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesHeLa
Cellular locationcell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Customer validation

WB(Rattus norvegicus, Homo sapiens, Schisandra chinensis, Mus musculus, Ctenopharyngodon idellus, Gallus gallus)

IF(Homo sapiens, Rattus norvegicus)

WB(Homo sapiens, Rattus norvegicus)

IF/ICC(Homo sapiens,Mus musculus)

IF(Rattus norvegicus)

Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb images

ABclonal:Western blot - Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854)}

Western blot - Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis of extracts of HeLa cells, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 antibody (AP0854) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PIK3R1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PIK3R1. (Distance between topics and target gene indicate popularity.) PIK3R1

* Data provided by citexs.com, for reference only.

Publishing research using AP0854? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (2)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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