Western blot analysis of extracts of various cell lines, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 antibody (AP0854) at 1:1000 dilution.Hela and C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
|Product name||Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Positive samples||HeLa, C2C12|
WB(Homo sapiens, Rattus norvegicus, Schisandra chinensis, Mus musculus)
* For research use only. Not for therapeutic or diagnostic purposes.
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