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Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Publications (7) Datasheet

ABclonal:Western blot - Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis of extracts of various cell lines, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 antibody (AP0854) at 1:1000 dilution.Hela and C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product namePhospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb
Catalog No.AP0854
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
ImmunogenA synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1).
SequenceRLYEE
Gene ID5295/5296/8503
Swiss protP27986/O00459/Q92569
SynonymsPIK3R1/PIK3R2/PIK3R3
Calculated MW42-54kDa/81-84kDa
Observed MW85KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, C2C12
Cellular location
Customer validation

WB(Homo sapiens, Rattus norvegicus, Schisandra chinensis, Mus musculus)

    ABclonal:Western blot - Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854) }

    Western blot - Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854)

    Western blot analysis of extracts of various cell lines, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 antibody (AP0854) at 1:1000 dilution.Hela and C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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