Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Publications (45) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis lysates from C2C12, using Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

You may also interested in:

Overview
Product name Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb
Catalog No. AP0854
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Immunogen information
Immunogen A synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1).
Sequence DRLYEE
Gene ID 529552968503
Swiss prot P27986O00459Q92569
Synonyms PIK3R1/PIK3R2/PIK3R3; Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464
Calculated MW 42-54kDa/81-84kDa
Observed MW 60kDa(P55)/85kDa(P85)

Applications
Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, 293T-SRC(Y530F)-Flag(N)
Cellular location cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Customer validation

WB (Rattus norvegicus, Homo sapiens, Schisandra chinensis, Mus musculus, Ctenopharyngodon idellus, Gallus gallus)

IF (Homo sapiens, Rattus norvegicus)

WB (Homo sapiens, Rattus norvegicus)

IF/ICC (Homo sapiens,Mus musculus)

IF (Rattus norvegicus)

WB，IHC，IF (Homo sapiens)

Documents
Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb images
ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)}

Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)}

Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis lysates from C2C12, using Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Inquire About This Product

Submit your question about AP0854 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on PIK3R1. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to PIK3R1. (Distance between topics and target gene indicate popularity.) PIK3R1

* Data provided by citexs.com, for reference only.

Publishing research using AP0854? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Add to Cart

or Request quote for bulk purchase

Related Products

Antibodies (2)

Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199 Rabbit pAb
AP0427

WB

Reactivity:Human, Mouse, Rat

Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199 Rabbit pAb

PI3 Kinase p85 alpha/beta/gamma mAb
A22996

ELISAWBIF/ICC

Reactivity:Mouse, Rat

PI3 Kinase p85 alpha/beta/gamma mAb

Secondary Antibodies (22)

HRP Goat Anti-Rabbit IgG (H+L)
AS014

ELISAWBIHC-P

HRP Goat Anti-Rabbit IgG (H+L)

FITC Goat Anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC Goat Anti-Rabbit IgG (H+L)

Cy3 Goat Anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3 Goat Anti-Rabbit IgG (H+L)
View All Related Products
Contact us to order