Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PEA15-S104 Rabbit pAb (AP0874)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PEA15-S104 Rabbit pAb (AP0874)

Western blot analysis of various lysates using Phospho-PEA15-S104 Rabbit pAb (AP0874) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name Phospho-PEA15-S104 Rabbit pAb
Catalog No. AP0874
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
Sequence IPSAK
Gene ID 8682
Swiss prot Q15121
Synonyms PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15; Phospho-PEA15-S104
Calculated MW 15kDa
Observed MW 15kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples C6, C6+PMA
Cellular location Cytoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-PEA15-S104 Rabbit pAb images

ABclonal:Western blot - Phospho-PEA15-S104 Rabbit pAb (AP0874)}

Western blot - Phospho-PEA15-S104 Rabbit pAb (AP0874)

Western blot analysis of various lysates using Phospho-PEA15-S104 Rabbit pAb (AP0874) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PEA15. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PEA15. (Distance between topics and target gene indicate popularity.) PEA15

* Data provided by citexs.com, for reference only.

Publishing research using AP0874? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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