Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PDGFRB-Y751 Rabbit pAb (AP0493)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-PDGFRB-Y751 Rabbit pAb (AP0493)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y751 antibody (AP0493) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name Phospho-PDGFRB-Y751 Rabbit pAb
Catalog No. AP0493
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y751 of human PDGFRB (NP_002600.1).
Sequence VDYVP
Gene ID 5159
Swiss prot P09619
Synonyms IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1; Phospho-PDGFRB-Y751
Calculated MW 124kDa
Observed MW 190kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3
Cellular location Cell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein
Customer validation

WB (Mus musculus)

Research Area

Phospho-PDGFRB-Y751 Rabbit pAb images

ABclonal:Western blot - Phospho-PDGFRB-Y751 Rabbit pAb (AP0493)}

Western blot - Phospho-PDGFRB-Y751 Rabbit pAb (AP0493)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y751 antibody (AP0493) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PDGFRB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PDGFRB. (Distance between topics and target gene indicate popularity.) PDGFRB

* Data provided by citexs.com, for reference only.

Publishing research using AP0493? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

Proteins (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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