Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-PAK1-T212 Rabbit pAb (AP0555)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-PAK1-T212 Rabbit pAb (AP0555)

Western blot analysis of extracts of various cell lines, using Phospho-PAK1-T212 pAb (AP0555) at 1:1000 dilution.293T cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name Phospho-PAK1-T212 Rabbit pAb
Catalog No. AP0555
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T212 of human PAK1 (NP_002567.3).
Sequence PVTPT
Gene ID 5058
Swiss prot Q13153
Synonyms IDDMSSD; p65-PAK; PAKalpha; alpha-PAK; Phospho-PAK1-T212
Calculated MW 61kDa
Observed MW 55kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T
Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasm, focal adhesion, invadopodium, ruffle membrane

Research Area

Phospho-PAK1-T212 Rabbit pAb images

ABclonal:Western blot - Phospho-PAK1-T212 Rabbit pAb (AP0555)}

Western blot - Phospho-PAK1-T212 Rabbit pAb (AP0555)

Western blot analysis of extracts of various cell lines, using Phospho-PAK1-T212 pAb (AP0555) at 1:1000 dilution.293T cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PAK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PAK1. (Distance between topics and target gene indicate popularity.) PAK1

* Data provided by citexs.com, for reference only.

Publishing research using AP0555? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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